至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

The effect of local delivery of adiponectin from biodegradable microsphere-scaffold composites on new bone formation in adiponectin knockout mice

J Mater Chem B. 2016-06-01; 
Dan Li, Yuan Guo, Hui Lu, Ren Wang, Hong-Cheng Hu, Song-He Lu, Xue-Fen Li, Zi-Chen Li, Yu-Wei Wu, Zhi-Hui Tang
Products/Services Used Details Operation
Recombinant Proteins … His-gAPN was then purified using a GE Pharmacia AKTA Purifier 10 (Ramsey, MN, USA) followed by endotoxin removal using a ToxinEraser™ Endotoxin Removal Kit L00338 (GenScript, Piscataway, NJ, USA) and isolation using Zeba Spin Desalting Columns, 7k MWCO, 10 … Get A Quote

摘要

Adiponectin (APN) is the most abundant adipocyte-secreted adipokine; it regulates energy homeostasis and exerts well-characterized insulin-sensitizing properties. Previous studies have verified that globular adiponectin (gAPN) is also involved in bone metabolism, although observations have been controversial. The purpose of the current study is to use an APN-knockout (APN-KO) mouse model to evaluate the local delivery of gAPN to new bone formation. Using chitosan microspheres (CMs), we found that following an initial burst at 1 week, the release behavior of gAPN from the scaffold was sustained in a linear manner for the first 4 weeks, followed by a slower, more stable release from week 5 onwards. Interestingly,... More

关键词